A Poplar Rust Effector Protein Associates with Protein Disulfide Isomerase and Enhances Plant Susceptibility.
Identifieur interne : 000238 ( Main/Exploration ); précédent : 000237; suivant : 000239A Poplar Rust Effector Protein Associates with Protein Disulfide Isomerase and Enhances Plant Susceptibility.
Auteurs : Mst Hur Madina [Canada] ; Md Saifur Rahman [Canada] ; Xiaoqiang Huang [États-Unis] ; Yang Zhang [États-Unis] ; Huanquan Zheng [Canada] ; Hugo Germain [Canada]Source :
- Biology [ 2079-7737 ] ; 2020.
Abstract
Melampsora larici-populina (Mlp), the causal agent of Populus leaf rust, secretes an array of effectors into the host through the haustorium to gain nutrients and suppress immunity. The precise mechanisms by which these effectors promote virulence remain unclear. To address this question, we developed a transgenic Arabidopsis line expressing a candidate effector, Mlp124357. Constitutive expression of the effector increased plant susceptibility to pathogens. A GxxxG motif present in Mlp124357 is required for its subcellular localization at the vacuolar membrane of the plant cell, as replacement of the glycine residues with alanines led to the delocalization of Mlp124357 to the nucleus and cytoplasm. We used immunoprecipitation and mass spectrometry (MS) to identify Mlp124357 interaction partners. Only one of the putative interaction partners knock-out line caused delocalization of the effector, indicating that Arabidopsis protein disulfide isomerase-11 (AtPDI-11) is required for the effector localization. This interaction was further confirmed by a complementation test, a yeast-two hybrid assay and a molecular modeling experiment. Moreover, localization results and infection assays suggest that AtPDI-11 act as a helper for Mlp124357. In summary, our findings established that one of Mlp effectors resides at the vacuole surface and modulates plant susceptibility.
DOI: 10.3390/biology9090294
PubMed: 32947987
PubMed Central: PMC7564345
Affiliations:
- Canada, États-Unis
- Michigan, Québec
- Montréal, Trois-Rivières
- Université McGill, Université du Québec à Trois-Rivières
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Poplar Rust Effector Protein Associates with Protein Disulfide Isomerase and Enhances Plant Susceptibility.</title><author><name sortKey="Madina, Mst Hur" sort="Madina, Mst Hur" uniqKey="Madina M" first="Mst Hur" last="Madina">Mst Hur Madina</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7</wicri:regionArea><orgName type="university">Université du Québec à Trois-Rivières</orgName><placeName><settlement type="city">Trois-Rivières</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Rahman, Md Saifur" sort="Rahman, Md Saifur" uniqKey="Rahman M" first="Md Saifur" last="Rahman">Md Saifur Rahman</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7</wicri:regionArea><orgName type="university">Université du Québec à Trois-Rivières</orgName><placeName><settlement type="city">Trois-Rivières</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Huang, Xiaoqiang" sort="Huang, Xiaoqiang" uniqKey="Huang X" first="Xiaoqiang" last="Huang">Xiaoqiang Huang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Computational Medicine and Bioinformatics, University of Michigan, 100 Washtenaw Avenue, Ann Arbor, MI 48109, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Computational Medicine and Bioinformatics, University of Michigan, 100 Washtenaw Avenue, Ann Arbor, MI 48109</wicri:regionArea><placeName><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Zhang, Yang" sort="Zhang, Yang" uniqKey="Zhang Y" first="Yang" last="Zhang">Yang Zhang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Computational Medicine and Bioinformatics, University of Michigan, 100 Washtenaw Avenue, Ann Arbor, MI 48109, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Computational Medicine and Bioinformatics, University of Michigan, 100 Washtenaw Avenue, Ann Arbor, MI 48109</wicri:regionArea><placeName><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Zheng, Huanquan" sort="Zheng, Huanquan" uniqKey="Zheng H" first="Huanquan" last="Zheng">Huanquan Zheng</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biology, McGill University, 1205 Dr. Penfield Avenue, Montreal, QC H3A 1B1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, McGill University, 1205 Dr. Penfield Avenue, Montreal, QC H3A 1B1</wicri:regionArea><orgName type="university">Université McGill</orgName><placeName><settlement type="city">Montréal</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Germain, Hugo" sort="Germain, Hugo" uniqKey="Germain H" first="Hugo" last="Germain">Hugo Germain</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7</wicri:regionArea><orgName type="university">Université du Québec à Trois-Rivières</orgName><placeName><settlement type="city">Trois-Rivières</settlement><region type="state">Québec</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32947987</idno><idno type="pmid">32947987</idno><idno type="doi">10.3390/biology9090294</idno><idno type="pmc">PMC7564345</idno><idno type="wicri:Area/Main/Corpus">000102</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000102</idno><idno type="wicri:Area/Main/Curation">000102</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000102</idno><idno type="wicri:Area/Main/Exploration">000102</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A Poplar Rust Effector Protein Associates with Protein Disulfide Isomerase and Enhances Plant Susceptibility.</title><author><name sortKey="Madina, Mst Hur" sort="Madina, Mst Hur" uniqKey="Madina M" first="Mst Hur" last="Madina">Mst Hur Madina</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7</wicri:regionArea><orgName type="university">Université du Québec à Trois-Rivières</orgName><placeName><settlement type="city">Trois-Rivières</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Rahman, Md Saifur" sort="Rahman, Md Saifur" uniqKey="Rahman M" first="Md Saifur" last="Rahman">Md Saifur Rahman</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7</wicri:regionArea><orgName type="university">Université du Québec à Trois-Rivières</orgName><placeName><settlement type="city">Trois-Rivières</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Huang, Xiaoqiang" sort="Huang, Xiaoqiang" uniqKey="Huang X" first="Xiaoqiang" last="Huang">Xiaoqiang Huang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Computational Medicine and Bioinformatics, University of Michigan, 100 Washtenaw Avenue, Ann Arbor, MI 48109, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Computational Medicine and Bioinformatics, University of Michigan, 100 Washtenaw Avenue, Ann Arbor, MI 48109</wicri:regionArea><placeName><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Zhang, Yang" sort="Zhang, Yang" uniqKey="Zhang Y" first="Yang" last="Zhang">Yang Zhang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Computational Medicine and Bioinformatics, University of Michigan, 100 Washtenaw Avenue, Ann Arbor, MI 48109, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Computational Medicine and Bioinformatics, University of Michigan, 100 Washtenaw Avenue, Ann Arbor, MI 48109</wicri:regionArea><placeName><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Zheng, Huanquan" sort="Zheng, Huanquan" uniqKey="Zheng H" first="Huanquan" last="Zheng">Huanquan Zheng</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biology, McGill University, 1205 Dr. Penfield Avenue, Montreal, QC H3A 1B1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, McGill University, 1205 Dr. Penfield Avenue, Montreal, QC H3A 1B1</wicri:regionArea><orgName type="university">Université McGill</orgName><placeName><settlement type="city">Montréal</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Germain, Hugo" sort="Germain, Hugo" uniqKey="Germain H" first="Hugo" last="Germain">Hugo Germain</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7</wicri:regionArea><orgName type="university">Université du Québec à Trois-Rivières</orgName><placeName><settlement type="city">Trois-Rivières</settlement><region type="state">Québec</region></placeName></affiliation></author></analytic><series><title level="j">Biology</title><idno type="ISSN">2079-7737</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><i>Melampsora larici-populina (Mlp)</i>, the causal agent of <i>Populus</i> leaf rust, secretes an array of effectors into the host through the haustorium to gain nutrients and suppress immunity. The precise mechanisms by which these effectors promote virulence remain unclear. To address this question, we developed a transgenic <i>Arabidopsis</i> line expressing a candidate effector, Mlp124357. Constitutive expression of the effector increased plant susceptibility to pathogens. A GxxxG motif present in Mlp124357 is required for its subcellular localization at the vacuolar membrane of the plant cell, as replacement of the glycine residues with alanines led to the delocalization of Mlp124357 to the nucleus and cytoplasm. We used immunoprecipitation and mass spectrometry (MS) to identify Mlp124357 interaction partners. Only one of the putative interaction partners knock-out line caused delocalization of the effector, indicating that <i>Arabidopsis</i> protein disulfide isomerase-11 (AtPDI-11) is required for the effector localization. This interaction was further confirmed by a complementation test, a yeast-two hybrid assay and a molecular modeling experiment. Moreover, localization results and infection assays suggest that AtPDI-11 act as a helper for Mlp124357. In summary, our findings established that one of <i>Mlp</i> effectors resides at the vacuole surface and modulates plant susceptibility.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32947987</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>27</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2079-7737</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><Issue>9</Issue><PubDate><Year>2020</Year><Month>Sep</Month><Day>16</Day></PubDate></JournalIssue><Title>Biology</Title><ISOAbbreviation>Biology (Basel)</ISOAbbreviation></Journal><ArticleTitle>A Poplar Rust Effector Protein Associates with Protein Disulfide Isomerase and Enhances Plant Susceptibility.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E294</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/biology9090294</ELocationID><Abstract><AbstractText><i>Melampsora larici-populina (Mlp)</i>, the causal agent of <i>Populus</i> leaf rust, secretes an array of effectors into the host through the haustorium to gain nutrients and suppress immunity. The precise mechanisms by which these effectors promote virulence remain unclear. To address this question, we developed a transgenic <i>Arabidopsis</i> line expressing a candidate effector, Mlp124357. Constitutive expression of the effector increased plant susceptibility to pathogens. A GxxxG motif present in Mlp124357 is required for its subcellular localization at the vacuolar membrane of the plant cell, as replacement of the glycine residues with alanines led to the delocalization of Mlp124357 to the nucleus and cytoplasm. We used immunoprecipitation and mass spectrometry (MS) to identify Mlp124357 interaction partners. Only one of the putative interaction partners knock-out line caused delocalization of the effector, indicating that <i>Arabidopsis</i> protein disulfide isomerase-11 (AtPDI-11) is required for the effector localization. This interaction was further confirmed by a complementation test, a yeast-two hybrid assay and a molecular modeling experiment. Moreover, localization results and infection assays suggest that AtPDI-11 act as a helper for Mlp124357. In summary, our findings established that one of <i>Mlp</i> effectors resides at the vacuole surface and modulates plant susceptibility.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Madina</LastName><ForeName>Mst Hur</ForeName><Initials>MH</Initials><AffiliationInfo><Affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rahman</LastName><ForeName>Md Saifur</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Xiaoqiang</ForeName><Initials>X</Initials><Identifier Source="ORCID">0000-0002-1005-848X</Identifier><AffiliationInfo><Affiliation>Department of Computational Medicine and Bioinformatics, University of Michigan, 100 Washtenaw Avenue, Ann Arbor, MI 48109, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Yang</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Computational Medicine and Bioinformatics, University of Michigan, 100 Washtenaw Avenue, Ann Arbor, MI 48109, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Huanquan</ForeName><Initials>H</Initials><Identifier Source="ORCID">0000-0003-2986-725X</Identifier><AffiliationInfo><Affiliation>Department of Biology, McGill University, 1205 Dr. Penfield Avenue, Montreal, QC H3A 1B1, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Germain</LastName><ForeName>Hugo</ForeName><Initials>H</Initials><Identifier Source="ORCID">0000-0002-7046-6194</Identifier><AffiliationInfo><Affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>950-231790</GrantID><Agency>Canada Research Chairs</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>09</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Biology (Basel)</MedlineTA><NlmUniqueID>101587988</NlmUniqueID><ISSNLinking>2079-7737</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">GxxxG motif</Keyword><Keyword MajorTopicYN="N">effector</Keyword><Keyword MajorTopicYN="N">fungal rust</Keyword><Keyword MajorTopicYN="N">helper protein</Keyword><Keyword MajorTopicYN="N">plant susceptibility</Keyword><Keyword MajorTopicYN="N">protein disulfide isomerase</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>07</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>09</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>09</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>9</Month><Day>19</Day><Hour>1</Hour><Minute>2</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>9</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>9</Month><Day>20</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32947987</ArticleId><ArticleId IdType="pii">biology9090294</ArticleId><ArticleId IdType="doi">10.3390/biology9090294</ArticleId><ArticleId IdType="pmc">PMC7564345</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>PLoS Pathog. 2015 Aug 28;11(8):e1005139</Citation><ArticleIdList><ArticleId IdType="pubmed">26317500</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2012 Mar;25(3):279-93</Citation><ArticleIdList><ArticleId IdType="pubmed">22046958</ArticleId></ArticleIdList></Reference><Reference><Citation>Photosynth Res. 2010 Apr;104(1):41-8</Citation><ArticleIdList><ArticleId IdType="pubmed">20012201</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2006 Feb;18(2):502-14</Citation><ArticleIdList><ArticleId IdType="pubmed">16399801</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2005 Apr 22;308(5721):548-50</Citation><ArticleIdList><ArticleId IdType="pubmed">15746386</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Microbiol. 2006 Sep;61(6):1485-96</Citation><ArticleIdList><ArticleId IdType="pubmed">16968222</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2011;6(9):e24657</Citation><ArticleIdList><ArticleId IdType="pubmed">21949741</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2008 Oct;228(5):851-62</Citation><ArticleIdList><ArticleId IdType="pubmed">18649087</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2010 Feb 12;327(5967):804-5</Citation><ArticleIdList><ArticleId IdType="pubmed">20150482</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2019 Nov;101(4-5):343-354</Citation><ArticleIdList><ArticleId IdType="pubmed">31621005</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2013 Aug 16;341(6147):746-51</Citation><ArticleIdList><ArticleId IdType="pubmed">23950531</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2011 Oct;24(10):1179-88</Citation><ArticleIdList><ArticleId IdType="pubmed">21635141</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2012;7(1):e29847</Citation><ArticleIdList><ArticleId IdType="pubmed">22238666</ArticleId></ArticleIdList></Reference><Reference><Citation>Biomolecules. 2013 Oct 21;3(4):848-69</Citation><ArticleIdList><ArticleId IdType="pubmed">24970193</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol Biochem. 2013 Sep;70:261-8</Citation><ArticleIdList><ArticleId IdType="pubmed">23800661</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2006 Dec 12;103(50):19200-5</Citation><ArticleIdList><ArticleId IdType="pubmed">17148606</ArticleId></ArticleIdList></Reference><Reference><Citation>J Pathog. 2011;2011:716041</Citation><ArticleIdList><ArticleId IdType="pubmed">22567338</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Microbiol. 2009 Feb;12(1):53-60</Citation><ArticleIdList><ArticleId IdType="pubmed">19168384</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2006 Aug;19(8):854-63</Citation><ArticleIdList><ArticleId IdType="pubmed">16903351</ArticleId></ArticleIdList></Reference><Reference><Citation>J Cell Physiol. 2002 Nov;193(2):154-63</Citation><ArticleIdList><ArticleId IdType="pubmed">12384992</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2015 Jun;28(6):689-700</Citation><ArticleIdList><ArticleId IdType="pubmed">25650830</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2008 Aug;21(8):1015-26</Citation><ArticleIdList><ArticleId IdType="pubmed">18616398</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2012 Jan;69(2):252-65</Citation><ArticleIdList><ArticleId IdType="pubmed">21914011</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2016 Feb;29(2):96-108</Citation><ArticleIdList><ArticleId IdType="pubmed">26646532</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17421-6</Citation><ArticleIdList><ArticleId IdType="pubmed">20847293</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2013 Mar 1;29(5):661-3</Citation><ArticleIdList><ArticleId IdType="pubmed">23325622</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Methods. 2015 Jan;12(1):7-8</Citation><ArticleIdList><ArticleId IdType="pubmed">25549265</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2012 Jan;8(1):e1002467</Citation><ArticleIdList><ArticleId IdType="pubmed">22241993</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2012 Dec 06;3:275</Citation><ArticleIdList><ArticleId IdType="pubmed">23230447</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2016 Apr;29(4):247-57</Citation><ArticleIdList><ArticleId IdType="pubmed">26713351</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2000 May 12;101(4):353-63</Citation><ArticleIdList><ArticleId IdType="pubmed">10830163</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2007 Dec;10(6):580-6</Citation><ArticleIdList><ArticleId IdType="pubmed">17884715</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Host Microbe. 2010 Feb 18;7(2):164-75</Citation><ArticleIdList><ArticleId IdType="pubmed">20159621</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2008 Aug;20(8):2205-20</Citation><ArticleIdList><ArticleId IdType="pubmed">18676877</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2006 Jul 1;34(Web Server issue):W310-4</Citation><ArticleIdList><ArticleId IdType="pubmed">16845016</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2013 Jan;87(1):464-73</Citation><ArticleIdList><ArticleId IdType="pubmed">23097431</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Protoc Bioinformatics. 2015 Dec 17;52:5.8.1-5.8.15</Citation><ArticleIdList><ArticleId IdType="pubmed">26678386</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2018 Oct 3;8(1):14718</Citation><ArticleIdList><ArticleId IdType="pubmed">30283062</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2016 Feb;29(2):83-95</Citation><ArticleIdList><ArticleId IdType="pubmed">26554735</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2018 Oct;30(10):2594-2615</Citation><ArticleIdList><ArticleId IdType="pubmed">30150314</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cells. 2011 Nov;32(5):459-75</Citation><ArticleIdList><ArticleId IdType="pubmed">21909944</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2010 Oct;23(10):1275-86</Citation><ArticleIdList><ArticleId IdType="pubmed">20831407</ArticleId></ArticleIdList></Reference><Reference><Citation>Cold Spring Harb Symp Quant Biol. 2012;77:235-47</Citation><ArticleIdList><ArticleId IdType="pubmed">23223409</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2009 Jan;22(1):18-30</Citation><ArticleIdList><ArticleId IdType="pubmed">19061399</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2010 Sep;187(4):1018-33</Citation><ArticleIdList><ArticleId IdType="pubmed">20636323</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Genet. 2010 Aug;11(8):539-48</Citation><ArticleIdList><ArticleId IdType="pubmed">20585331</ArticleId></ArticleIdList></Reference><Reference><Citation>CSH Protoc. 2007 Jan 01;2007:pdb.prot4683</Citation><ArticleIdList><ArticleId IdType="pubmed">21356935</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2015 Jan;81(1):40-52</Citation><ArticleIdList><ArticleId IdType="pubmed">25284001</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2002 May;7(5):193-5</Citation><ArticleIdList><ArticleId IdType="pubmed">11992820</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chem. 2003 Sep 1;75(17):4646-58</Citation><ArticleIdList><ArticleId IdType="pubmed">14632076</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13544-9</Citation><ArticleIdList><ArticleId IdType="pubmed">20624958</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2014 Jun;78(6):1003-13</Citation><ArticleIdList><ArticleId IdType="pubmed">24684167</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2006 Nov 16;444(7117):323-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17108957</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2014 Aug;20:96-103</Citation><ArticleIdList><ArticleId IdType="pubmed">24879450</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 2015 Aug 25;54(33):5125-35</Citation><ArticleIdList><ArticleId IdType="pubmed">26244771</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2001 Jun;26(5):523-34</Citation><ArticleIdList><ArticleId IdType="pubmed">11439138</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2017 Feb;12(2):255-278</Citation><ArticleIdList><ArticleId IdType="pubmed">28079879</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2006;1(4):2019-25</Citation><ArticleIdList><ArticleId IdType="pubmed">17487191</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2012 Dec;25(12):1523-30</Citation><ArticleIdList><ArticleId IdType="pubmed">22876960</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2003 Jul;13(7):1675-85</Citation><ArticleIdList><ArticleId IdType="pubmed">12840044</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Microbiol. 2016 Apr;18(4):453-65</Citation><ArticleIdList><ArticleId IdType="pubmed">26426202</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2011 May 31;108(22):9166-71</Citation><ArticleIdList><ArticleId IdType="pubmed">21536894</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2018 Jan;19(1):191-200</Citation><ArticleIdList><ArticleId IdType="pubmed">27868319</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W363-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15980490</ArticleId></ArticleIdList></Reference><Reference><Citation>Proteins. 2012 Jul;80(7):1715-35</Citation><ArticleIdList><ArticleId IdType="pubmed">22411565</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Canada</li><li>États-Unis</li></country><region><li>Michigan</li><li>Québec</li></region><settlement><li>Montréal</li><li>Trois-Rivières</li></settlement><orgName><li>Université McGill</li><li>Université du Québec à Trois-Rivières</li></orgName></list><tree><country name="Canada"><region name="Québec"><name sortKey="Madina, Mst Hur" sort="Madina, Mst Hur" uniqKey="Madina M" first="Mst Hur" last="Madina">Mst Hur Madina</name></region><name sortKey="Germain, Hugo" sort="Germain, Hugo" uniqKey="Germain H" first="Hugo" last="Germain">Hugo Germain</name><name sortKey="Rahman, Md Saifur" sort="Rahman, Md Saifur" uniqKey="Rahman M" first="Md Saifur" last="Rahman">Md Saifur Rahman</name><name sortKey="Zheng, Huanquan" sort="Zheng, Huanquan" uniqKey="Zheng H" first="Huanquan" last="Zheng">Huanquan Zheng</name></country><country name="États-Unis"><region name="Michigan"><name sortKey="Huang, Xiaoqiang" sort="Huang, Xiaoqiang" uniqKey="Huang X" first="Xiaoqiang" last="Huang">Xiaoqiang Huang</name></region><name sortKey="Zhang, Yang" sort="Zhang, Yang" uniqKey="Zhang Y" first="Yang" last="Zhang">Yang Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PlantPathoEffV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000238 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000238 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PlantPathoEffV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32947987
|texte= A Poplar Rust Effector Protein Associates with Protein Disulfide Isomerase and Enhances Plant Susceptibility.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32947987" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PlantPathoEffV1
| This area was generated with Dilib version V0.6.38. |  |